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پپتیدهای آنتی بادی

» پپتیدها » پپتیدهای آنتی بادی

  • مشخصات فنی
  • Single Antibody Service
  • Package Antibody Service
  • Advantages

Featured Custom Antibody Services
Antigen
Antigen peptide design
Peptide synthesis
Protein expression and purification
Antigen conjugation (to KLH, BSA or OVA)
Animals
Rabbit, mouse, rat, chicken, guinea pig, goat etc.
Polyclonal antibody service
Application guaranteed antibody (ELISA, WB, IHC etc.)
Modification-specific antibody (Phospho-, acetyl-, متیل- antibody, etc.)
Monoclonal antibody service
Application guaranteed antibody (ELISA, WB, IHC etc.)
Modification-specific antibody (Phospho-, acetyl-, متیل- antibody, etc.)
Antibody purification
Protein A/G/L purification
Antigen affinity purification
Antibody identification
ELISA
Dot blot
Western blot
IHC
Antibody labeling
HRP
Biotin
FITC

Peptide service

Service Items Time
Peptide design (suggestion) 1-2 روزها
Peptide synthesis 2 هفته ها
Peptide conjugation (KLH/BSA/OVA,≤5mg) 2-3 روزها

Protein service

Service Items Time
Gene synthesis 3-4 هفته ها
Gene amplification (Template provided by customer,≤1000bp) 2-3 هفته ها
Vector construction 2-3 هفته ها
recombinant selection 1 week
protein expression and purification 2-3 هفته ها

Polyclonal antibody service

Service Items Time
Protein checked by SDS-PAGE 2-3 روزها
Pre-immune serum collection 1 day
Immunization & serum collection (2 rabbits) 10 هفته ها
Immunization & serum collection (2 rabbits, Fast) 6-8 هفته ها
ELISA identification 2-3 روزها
Prescreen (ELISA) 2-3 روزها
Prescreen (Western Blot) 1 week
Prescreen (Dot Blot) 1 week
Additional rabbit (1 rabbit) 6-10 هفته ها
Animal maintenance (1 rabbit) 1 week
Additional bleed (10ml, 1 rabbit) 1 week
Extension boost and bleed (10ml, 1 rabbit) 1week
Immunization & serum collection (1 goat) 20 هفته ها
Immunization & serum collection (2 rats) 10 هفته ها
Immunization & serum collection (4 موش) 10 هفته ها
Immunization & serum collection (2 guinea pigs) 11 هفته ها

Monoclonal antibody service

Service Items Time
Protein checked by SDS-PAGE 2-3 روزها
Pre-immune serum collection 1 day
Immunization & serum collection (4 موش) 6-10 هفته ها
ELISA identification 2-3 روزها
Fusion and hybridoma development 10-16 هفته ها
Isotype determination 1 week
Ascites production (3 موش) 3-4 هفته ها
Roller bottle cell culture (1L) 5-7 هفته ها
Prescreen (ELISA) 2-3 روزها
Prescreen (Western Blot) 1 week
Prescreen (Dot Blot) 1 week
Clone maintenance (1 clone) 1 week
Clone storage (1 clone) 1week

Antibody purification service

Service Items Time
Saturated ammonium sulfate precipitation (≤60ml) 3-5روزها
Protein A/G affinity purification (≤60ml) 3-5 روزها
Antigen affinity column preparation 2-3 روزها
Antigen affinity purification (≤60ml) 3-5 روزها

Antibody identification service

Service Items Time
ELISA identification (1-10 samples) 2-3 روزها
Cell extraction (1 sample) 2-3 روزها
Tissue extraction (1 sample) 2-3 روزها
Protein concentration assay (1 sample) 2-3 روزها
Western Blot identification (1-8 samples) 1 week
Dot Blot identification (1-10 samples) 2-3 روزها
IHC identification (section provided by customer,1 photo) 1 week

Antibody labeling service

Service Items Time
HRP labeling (≤5mg) 1-2 هفته ها
Biotin labeling (≤5mg) 1-2 هفته ها
Polyclonal Antibody Service
Package Number شرح Package Contents Time
Polyclonal package 1

(From protein to antiserum)

l Customer provides antigen protein

l Protein will be checked by SDS-PAGE

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l 1-2ml pre-bleed

l 40-60ml post-bleed

l Project datasheet including ELISA data

10-12 هفته ها
Polyclonal package 2 (From protein to antigen purified antibody) l Customer provides antigen protein

l Protein will be checked by SDS-PAGE

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l Dot blot confirmation

l 1-2ml pre-bleed

l 2-5mg affinity purified antibody

l Project datasheet including ELISA and dot blot data

10-12 هفته ها
Polyclonal package 3 (From peptide design to antiserum) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l 2-3mg antigen peptide

l 1-2ml pre-bleed

l 40-60ml post-bleed

l Project datasheet including ELISA data

12-15 هفته ها
Polyclonal package 4 (From peptide design to antigen purified antibodies) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l Dot blot confirmation

l 2-3mg antigen peptide

l 1-2ml pre-bleed

l 2-5mg affinity purified antibody

l Project datasheet including ELISA and dot blot data

12-15 هفته ها
Polyclonal package 5 (From plasmid to antiserum) l Customer provides expression vector

l Express and purify the antigen protein

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l 1mg antigen protein

l 1-2ml pre-bleed

l 40-60ml post-bleed

l Project datasheet including ELISA data

12-15 هفته ها
Polyclonal package 6 (From plasmid to antigen purified antibody) l Customer provides expression vector

l Express and purify the antigen protein

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l Dot blot confirmation

l 1mg antigen protein

l 1-2ml pre-bleed

l 2-5mg affinity purified antibody

l Project datasheet including ELISA and dot blot data

12-15 هفته ها
Polyclonal package 7 (From cDNA to antiserum) l Customer provides protein information

l We clone the gene and construct the expression vector for fusion protein

l Express and purify the antigen protein

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Recombinant vector

l 1mg protein

l 1-2ml pre-bleed

l 40-60ml post-bleed

l Project datasheet including ELISA data

14-16 هفته ها
Polyclonal package 8 (From cDNA to antigen purified antibody) l Customer provides gene and protein information

l We clone the gene and construct the expression vector for fusion protein

l Express and purify the antigen protein

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l Dot blot confirmation

l Recombinant vector

l 1mg protein

l 1-2ml pre-bleed

l 2-5mg affinity purified antibody

l Project datasheet including ELISA and dot blot data

14-16 هفته ها
Polyclonal package 9 (Phospho-specific polyclonal antibody) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l 2-3mg peptide/EA (non- & phosphor- peptide)

l 1-2ml pre-bleed

l 2-5mg antibody against unphospho-peptide

l 2-5mg phospho-specific antibody

l Project datasheet including ELISA and dot blot data

13-16 هفته ها
Polyclonal package 10 (Lys mono-methylated specific polyclonal antibody) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l 2-3mg peptide/EA (non- & mono-methylated peptide)

l 1-2ml pre-bleed

l 2-5mg antibody against unmethylated peptide

l 2-5mg Lys(Me) specific antibody

l Project datasheet including ELISA and dot blot data

13-16 هفته ها
Polyclonal package 11 (Lys dimethylated specific polyclonal antibody) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l 2-3mg peptide/EA (non- & di-methylated peptide)

l 1-2ml pre-bleed

l 2-5mg antibody against unmethylated peptide

l 2-5mg Lys(Me2) specific antibody

l Project datasheet including ELISA and dot blot data

13-16 هفته ها
Polyclonal package 12 (Lys trimethylated specific polyclonal antibody) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l 2-3mg peptide/EA (non- & tri-methylated peptide)

l 1-2ml pre-bleed

l 2-5mg antibody against unmethylated peptide

l 2-5mg Lys(Me3) specific antibody

l Project datasheet including ELISA and dot blot data

13-16 هفته ها
Polyclonal package13 (Arg methylation-specific polyclonal antibody) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l 2-3mg peptide/EA (non- & methylated peptide)

l 1-2ml pre-bleed

l 2-5mg antibody against unmethylated peptide

l 2-5mg Arg(Me) specific antibody

l Project datasheet including ELISA and dot blot data

13-16 هفته ها
Polyclonal package14 (Arg di-methylation (Symmetry)-specific polyclonal antibody) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l 2-3mg peptide/EA (non- & dimethylated peptide)

l 1-2ml pre-bleed

l 2-5mg antibody against unmethylated peptide

l 2-5mg Arg(Me2) specific antibody

l Project datasheet including ELISA and dot blot data

13-16 هفته ها
Polyclonal package15 (Arg di-methylation (Asymmetric)-specific polyclonal antibody) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l 2-3mg peptide/EA (non- & dimethylated peptide)

l 1-2ml pre-bleed

l 2-5mg antibody against unmethylated peptide

l 2-5mg Arg(Me2) specific antibody

l Project datasheet including ELISA and dot blot data

13-16 هفته ها
Polyclonal package 16 (Western guaranteed polyclonal antibody) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l Western blot confirmation

l 2-3mg antigen peptide

l 1-2ml pre-bleed

l 2-5mg affinity purified antibody

l Project datasheet including ELISA and Western blot data

15-18 هفته ها
Polyclonal package17 (Western and IHC guaranteed polyclonal antibody) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize two New Zealand rabbits

l Titer the immunization by ELISA

l Post-bleed collection

l Antigen affinity purification

l WB and IHC confirmation

l 2-3mg antigen peptide

l 1-2ml pre-bleed

l 2-5mg affinity purified antibody

l Project datasheet including ELISA, Western blot and IHC data

15-18 هفته ها

 

 

Monoclonal Antibody Service
Package Number شرح Package Contents Time
Monoclonal package1 (From protein to ascites purification) l Customer provides antigen protein

l Protein will be checked by SDS-PAGE

l Immunize four mice (Balb/C)

l Titer the immunization by ELISA

l Hybridoma fusion and cloning

l Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones).

l IgG isotype determination

l Ascites are prepared from two hybridoma clones which customer selects.

l Antigen affinity purification

l Dot blot for QC.

l 50ul pre-bleed (1:10dilution)

l 2-5 hybridoma clones

l 2-5mg purified antibody from two hybridoma clones

l Project datasheet including ELISA, dot blot and isotype determination data

18-24 هفته ها
Monoclonal package2 (From peptide design to ascites purification) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize four mice (Balb/C)

l Titer the immunization by ELISA

l Hybridoma fusion and cloning

l Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones).

l IgG isotype determination

l Ascites are prepared from two hybridoma clones which customer selects.

l Antigen affinity purification

l Dot blot for QC.

l 2-3mg peptides

l 50ul pre-bleed (1:10dilution)

l 2-5 hybridoma clones

l 2-5mg purified antibody from two hybridoma clones

l Project datasheet including ELISA, dot blot and isotype determination data

20-26 هفته ها
Monoclonal package3 (From plasmid to ascites purification) l Customer provides expression vector

l Express and purify the antigen protein

l Immunize four mice (Balb/C)

l Titer the immunization by ELISA

l Hybridoma fusion and cloning

l Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones).

l IgG isotype determination

l Ascites are prepared from two hybridoma clones which customer selects.

l Antigen affinity purification

l Dot blot for QC.

l 1mg protein

l 50ul pre-bleed (1:10dilution)

l 2-5 hybridoma clones

l 2-5mg purified antibody from two hybridoma clones

l Project datasheet including ELISA, dot blot and isotype determination data

20-26 هفته ها
Monoclonal package4 (From cDNA to ascites purification) l Customer provides gene and protein information

l We clone the gene and construct the expression vector for fusion protein

l Express and purify the antigen protein

l Immunize four mice (Balb/C)

l Titer the immunization by ELISA

l Hybridoma fusion and cloning

l Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones).

l IgG isotype determination

l Ascites are prepared from two hybridoma clones which customer selects.

l Antigen affinity purification

l Dot blot for QC.

l recombinant vector

l 1mg protein

l 50ul pre-bleed (1:10dilution)

l 2-5 hybridoma clones

l 2-5mg purified antibody from two hybridoma clones

l Project datasheet including ELISA, dot blot and isotype determination data

22-26 هفته ها
Monoclonal package5 (Phospho- specific monoclonal antibodies) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize six mice (Balb/C)

l Titer the immunization by ELISA

l Hybridoma fusion and cloning

l Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones).

l IgG isotype determination

l Ascites are prepared from two hybridoma clones which customer selects.

l Antigen affinity purification

l Dot blot for QC.

l 2-3mg peptide including non- and phosphor-peptide

l 50ul pre-bleed (1:10dilution)

l 2-5 hybridoma clones specific for phosphor-peptide

l 2 hybridoma clones for non-phospho-peptide

l 2-5mg purified phosphor-specific antibody from two hybridoma clones

l 2-5mg purified non-specific antibody from two hybridoma clones

l Project datasheet including ELISA, dot blot and isotype determination data

21-26 هفته ها
Monoclonal package6 (Western guaranteed monoclonal antibodies) l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)

l Synthesize one peptide (12-22aa, purity>85%)

l Conjugate the peptide to carrier protein (KLH, BSA or OVA)

l Immunize six mice (Balb/C)

l Titer the immunization by ELISA

l Hybridoma fusion and cloning

l Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones).

l IgG isotype determination

l Ascites are prepared from two hybridoma clones which customer selects.

l Antigen affinity purification

l Western blot for QC.

l 2-3mg peptides

l 50ul pre-bleed (1:10dilution)

l 2-5 hybridoma clones

l 2-5mg purified antibody from two hybridoma clones

l Project datasheet including ELISA, Western blot and isotype determination data

20-26 هفته ها

Why Choose our Antibody Service?

Free consultation
We will analyze your protein and design the antigen peptide, and choose the most suitable package for your project.
Accurate antigen prediction.Combining genomics, proteomics and structure genomics, we have created an effective method for antigen design. We guarantee that our antibodies, generated from antigen peptide, can be applied in Western blot.

Experienced scientists and technicians
We have a wealth of experience for production of over 5000 antibodies. Technicians in our animal facility have undergone special training in animal breeding and immunization organized by America specialists.

Strict quality control

The standards of our antigen design, antigen purity and animal immunization are much higher than those of other companies.

Low price but high quality
We offer the highest quality and the best services with the most competitive price.

Strong technical support
We update project progress to our customers and confer with them regularly. Customers who order our custom antibody service will enjoy lifetime technical support.

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    واتس اپ: +8615636286252

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