| Polyclonal Antibody Service |
| Package Number |
Beschreibung |
Package Contents |
Time |
| Polyclonal package 1
(From protein to antiserum) |
l Customer provides antigen protein
l Protein will be checked by SDS-PAGE
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection |
l 1-2ml pre-bleed
l 40-60ml post-bleed
l Project datasheet including ELISA data |
10-12 Wochen |
| Polyclonal package 2 (From protein to antigen purified antibody) |
l Customer provides antigen protein
l Protein will be checked by SDS-PAGE
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification
l Dot blot confirmation |
l 1-2ml pre-bleed
l 2-5mg affinity purified antibody
l Project datasheet including ELISA and dot blot data |
10-12 Wochen |
| Polyclonal package 3 (From peptide design to antiserum) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, Reinheit>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection |
l 2-3mg antigen peptide
l 1-2ml pre-bleed
l 40-60ml post-bleed
l Project datasheet including ELISA data |
12-15 Wochen |
| Polyclonal package 4 (From peptide design to antigen purified antibodies) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, Reinheit>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification
l Dot blot confirmation |
l 2-3mg antigen peptide
l 1-2ml pre-bleed
l 2-5mg affinity purified antibody
l Project datasheet including ELISA and dot blot data |
12-15 Wochen |
| Polyclonal package 5 (From plasmid to antiserum) |
l Customer provides expression vector
l Express and purify the antigen protein
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection |
l 1mg antigen protein
l 1-2ml pre-bleed
l 40-60ml post-bleed
l Project datasheet including ELISA data |
12-15 Wochen |
| Polyclonal package 6 (From plasmid to antigen purified antibody) |
l Customer provides expression vector
l Express and purify the antigen protein
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification
l Dot blot confirmation |
l 1mg antigen protein
l 1-2ml pre-bleed
l 2-5mg affinity purified antibody
l Project datasheet including ELISA and dot blot data |
12-15 Wochen |
| Polyclonal package 7 (From cDNA to antiserum) |
l Customer provides protein information
l We clone the gene and construct the expression vector for fusion protein
l Express and purify the antigen protein
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection |
l Recombinant vector
l 1mg protein
l 1-2ml pre-bleed
l 40-60ml post-bleed
l Project datasheet including ELISA data |
14-16 Wochen |
| Polyclonal package 8 (From cDNA to antigen purified antibody) |
l Customer provides gene and protein information
l We clone the gene and construct the expression vector for fusion protein
l Express and purify the antigen protein
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification
l Dot blot confirmation |
l Recombinant vector
l 1mg protein
l 1-2ml pre-bleed
l 2-5mg affinity purified antibody
l Project datasheet including ELISA and dot blot data |
14-16 Wochen |
| Polyclonal package 9 (Phospho-specific polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, Reinheit>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
l 2-3mg peptide/EA (non- & phosphor- peptide)
l 1-2ml pre-bleed
l 2-5mg antibody against unphospho-peptide
l 2-5mg phospho-specific antibody
l Project datasheet including ELISA and dot blot data |
13-16 Wochen |
| Polyclonal package 10 (Lys mono-methylated specific polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, Reinheit>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
l 2-3mg peptide/EA (non- & mono-methylated peptide)
l 1-2ml pre-bleed
l 2-5mg antibody against unmethylated peptide
l 2-5mg Lys(Me) specific antibody
l Project datasheet including ELISA and dot blot data |
13-16 Wochen |
| Polyclonal package 11 (Lys dimethylated specific polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, Reinheit>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
l 2-3mg peptide/EA (non- & di-methylated peptide)
l 1-2ml pre-bleed
l 2-5mg antibody against unmethylated peptide
l 2-5mg Lys(Me2) specific antibody
l Project datasheet including ELISA and dot blot data |
13-16 Wochen |
| Polyclonal package 12 (Lys trimethylated specific polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, Reinheit>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
l 2-3mg peptide/EA (non- & tri-methylated peptide)
l 1-2ml pre-bleed
l 2-5mg antibody against unmethylated peptide
l 2-5mg Lys(Me3) specific antibody
l Project datasheet including ELISA and dot blot data |
13-16 Wochen |
| Polyclonal package13 (Arg methylation-specific polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, Reinheit>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
l 2-3mg peptide/EA (non- & methylated peptide)
l 1-2ml pre-bleed
l 2-5mg antibody against unmethylated peptide
l 2-5mg Arg(Me) specific antibody
l Project datasheet including ELISA and dot blot data |
13-16 Wochen |
| Polyclonal package14 (Arg di-methylation (Symmetry)-specific polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, Reinheit>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
l 2-3mg peptide/EA (non- & dimethylated peptide)
l 1-2ml pre-bleed
l 2-5mg antibody against unmethylated peptide
l 2-5mg Arg(Me2) specific antibody
l Project datasheet including ELISA and dot blot data |
13-16 Wochen |
| Polyclonal package15 (Arg di-methylation (Asymmetric)-specific polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, Reinheit>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
l 2-3mg peptide/EA (non- & dimethylated peptide)
l 1-2ml pre-bleed
l 2-5mg antibody against unmethylated peptide
l 2-5mg Arg(Me2) specific antibody
l Project datasheet including ELISA and dot blot data |
13-16 Wochen |
| Polyclonal package 16 (Western guaranteed polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, Reinheit>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification
l Western blot confirmation |
l 2-3mg antigen peptide
l 1-2ml pre-bleed
l 2-5mg affinity purified antibody
l Project datasheet including ELISA and Western blot data |
15-18 Wochen |
| Polyclonal package17 (Western and IHC guaranteed polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, Reinheit>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification
l WB and IHC confirmation |
l 2-3mg antigen peptide
l 1-2ml pre-bleed
l 2-5mg affinity purified antibody
l Project datasheet including ELISA, Western blot and IHC data |
15-18 Wochen |
